Possibly. But don’t take a chance. If your lab protocols do not include Cascade BioScience Monoclonal Anti-Human PTEN (clone 6H2.1), you may experience non-specific IHC and high-background western blots.6H2.1 has been the gold standard since 1999 and is the most specific PTEN antibody available to the research community today. This valuable tool has been cited in dozens of publications and used in multiple applications.

Pallares et al., (2005) report "6H2.1 was the only one that exhibited a correlation with the presence of molecular alterations in PTEN, and a statistically significant association with immunostaining for phosphorylated AKT. The [other commercial antibodies evaluated] produced positive staining in PTEN-deficient cell line blocks, suggesting nonspecific staining. The results indicate that monoclonal antibody 6H2.1 may be a suitable alternative for tumors with inactivation of PTEN." Mod. Pathol. 18:719-27, 2005.

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Description Catalog # Vial Size Price
Monoclonal Anti-Human PTEN (clone 6H2.1) ABM-2052 100 µg $379
>>SPECIAL OFFER Order 2 vials of monoclonal Anti-PTEN and receive a 3rd vial at NO CHARGE. Offer expires 3/31/06
Research Applications
Wester Immunoblotting
Immunoprecipitation
Immunocytochemistry
Immunofluorescence
ELISA
Immunohistochemical Analysis: Photomicrograph illustrates PTEN non- expressing neoplastic endometrial glands in a background of normal expressing glands and stroma.
Immunoblot Analysis:
6H2.1 single band specificity on 7 breast cancer cell lines; one of which is PTEN-null; one of which has a monoallelic PTEN deletion and a missense mutation
Antibody Characteristics
Reactivity: human, mouse; others likely

Specificity: single band-specific; detects PTEN at ~60 kDa in total cell lysates

Immunogen: full-length fusion protein; human sequence epitope in red:

Host/Ig Type: mouse monoclonal IgG

Purification: protein A chromatography

Quality control: by WB on numerous breast cancer cell lines

Liquid Carrier: phosphate-buffered saline

Storage/Stability: -20˚ for 2 years

Literature Citations
Click here for over 40 publications that cite the use of CBI Anti Human PTEN, clone 6H2.1
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