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Description Catalog # Vial Size Price (USD)
Anti-Human Phospho-AKT1/PKB (pThr308), polyclonal ABP-6003 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
Western Blot:
0.5 -1.0 micrograms/ml
Immunoprecipitation:
3-5 micrograms/sample derived from 1 x 10E6 cells
ELISA:
0.1-1 micrograms/ml
Reactivity:
human
Specificity:
reacts predominantly with AKT1; recognizes the phosphorylated Threonine 308 of AKT1, not other phosphorylated sites or non-phosphorylated AKT1
Immunogen:
synthetic peptide corresponding to AKT1/PKB alpha of human origin at the phosphorylated site of Threonine 308
Host/Ig Type:
rabbit polyclonal IgG
Purification:
peptide affinity purification
Quality Control:
evaluated with a dot blot assay using synthetically prepared AKT peptides
Liquid Carrier:
phosphate buffered saline (pH 7.4) containing 0.02% Proclin300.
Storage:
-20˚C
Stability:
2 years
About AKT
General References

1. Chan T et al (1999) Annu. Rev. Biochem. 68, 965-1014.
2. Cho H et al (2001) J. Biol. Chem, 276: 38349-38352.
3. Jones PF et al, (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171.
4. Cross DA et al (1995) Nature 378: 758.
5. Andjelkovic M et al (1996) Proc. Natl. Acad. Sci. U.S.A 93: 5699.