Copyright © 2005 Cascade BioScience, Inc. All rights reserved.
Description | Catalog # | Vial Size | Price (USD) |
Anti-Human Phospho-AKT1/PKB (pSer473), polyclonal | ABP-6004 | 100 ug | $295 |
Western Blot:
|
0.5 -1.0 micrograms/ml |
Immunoprecipitation:
|
3-5 micrograms/sample derived from 1 x 10E6 cells |
ELISA:
|
0.1-1 micrograms/ml |
Reactivity:
|
human |
Specificity:
|
reacts predominantly with AKT1; a ~60 kDa immunoreactive protein from stimulated 3T3 or A431 cell lysate is detected by immunoblot (samples are boiled prior to 10% SDS-PAGE).; band is abolished by the depletion of the synthetic phosphospecific peptide; recognizes only the phosphorylated Serine 473 of AKT1, not other phosphorylated sites or non-phosphorylated AKT1. |
Immunogen:
|
synthetic peptide corresponding to carboxyl terminus of AKT1/PKB alpha of human origin at the phosphorylated site of Serine 473 |
Host/Ig Type:
|
rabbit polyclonal IgG |
Purification:
|
peptide affinity purification |
Quality Control:
|
in addition to western blotting, tha antibody is also evaluated with a dot blot assay using synthetically prepared AKT peptides. |
Liquid Carrier:
|
phosphate buffered saline (pH 7.4) containing 0.02% Proclin300. |
Storage:
|
-20˚C |
Stability:
|
2 years |
1. Chan T et al (1999) Annu. Rev. Biochem. 68, 965-1014.
2. Cho H et al (2001) J. Biol. Chem, 276: 38349-38352.
3. Jones PF et al, (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171.
4. Cross DA et al (1995) Nature 378: 758.
5. Andjelkovic M et al (1996) Proc. Natl. Acad. Sci. U.S.A 93: 5699.
Western blot:
Analysis of AKT1 protein on the stimulated A431 cell lysate by anti-AKT1 (pSer473; ABP-6004) at 1.0 microgram/ml (lane 1) and depleted by Phosphoserine 473 peptide (lane 2)