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Description Catalog # Vial Size Price (USD)
Anti-Human Phospho-AKT1/PKB (pSer473), polyclonal ABP-6004 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
Western Blot:
0.5 -1.0 micrograms/ml
3-5 micrograms/sample derived from 1 x 10E6 cells
0.1-1 micrograms/ml
reacts predominantly with AKT1; a ~60 kDa immunoreactive protein from stimulated 3T3 or A431 cell lysate is detected by immunoblot (samples are boiled prior to 10% SDS-PAGE).; band is abolished by the depletion of the synthetic phosphospecific peptide; recognizes only the phosphorylated Serine 473 of AKT1, not other phosphorylated sites or non-phosphorylated AKT1.
synthetic peptide corresponding to carboxyl terminus of AKT1/PKB alpha of human origin at the phosphorylated site of Serine 473
Host/Ig Type:
rabbit polyclonal IgG
peptide affinity purification
Quality Control:
in addition to western blotting, tha antibody is also evaluated with a dot blot assay using synthetically prepared AKT peptides.
Liquid Carrier:
phosphate buffered saline (pH 7.4) containing 0.02% Proclin300.
2 years
About AKT
General References

1. Chan T et al (1999) Annu. Rev. Biochem. 68, 965-1014.
2. Cho H et al (2001) J. Biol. Chem, 276: 38349-38352.
3. Jones PF et al, (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171.
4. Cross DA et al (1995) Nature 378: 758.
5. Andjelkovic M et al (1996) Proc. Natl. Acad. Sci. U.S.A 93: 5699.

Western blot:
Analysis of AKT1 protein on the stimulated A431 cell lysate by anti-AKT1 (pSer473; ABP-6004) at 1.0 microgram/ml (lane 1) and depleted by Phosphoserine 473 peptide (lane 2)