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Description Catalog # Vial Size Price (USD)
Anti-Human Phospho-AKT1/PKB (paired 308), polyclonal ABP-6005 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
Western Blot:
0.5 -1.0 micrograms/ml
3-5 micrograms/sample derived from 1 x 10E6 cells
0.1-1 micrograms/ml
human, rat, mouse

reacts predominantly with AKT1. By immunoblot analysis, detect a ~60 kDa immunoreactive protein from different cell lysates and normal mouse/rat tissues in Westen immunoblots. This band is abolished by the depletion of the synthetic peptide. Samples are boiled prior to SDS-PAGE).

synthetic peptide corresponds to AKT1 surrounding Threonine 308,
Host/Ig Type:
rabbit polyclonal IgG
peptide affinity purification
Quality Control:
by western blot
Liquid Carrier:
phosphate buffered saline (pH 7.4) containing 0.02% Proclin300.
2 years
About AKT
General References

1. Chan T et al (1999) Annu. Rev. Biochem. 68, 965-1014.
2. Cho H et al (2001) J. Biol. Chem, 276: 38349-38352.
3. Jones PF et al, (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171.
4. Cross DA et al (1995) Nature 378: 758.
5. Andjelkovic M et al (1996) Proc. Natl. Acad. Sci. U.S.A 93: 5699.

Western immunoblot:
cell extracts derived from brain, heart, muscle, liver, spleen, thymus, testes, kidney and lung repectively probed with Anti-AKT1 (Paired 308; Cat. #ABP-6005)