Copyright © 2005 Cascade BioScience, Inc. All rights reserved.
Description | Catalog # | Vial Size | Price (USD) |
Anti-Human AKT3 (NT), polyclonal | ABP-6007 | 100 ug | $295 |
Western Blot:
|
0.5 -2.0 micrograms/ml |
Immunoprecipitation:
|
3-5 micrograms/sample derived from 1 x 10E6 cells |
ELISA:
|
0.1-1 micrograms/ml |
Reactivity:
|
human, rat, mouse |
Specificity:
|
reacts specifically with Akt3 at ~48 kDa; immunoreactive protein from LoVo, HeLa, or 3T3 cell lysates is detected by immunoblot; band is abolished by the addition of the immunizing Akt3 peptide |
Immunogen:
|
synthetic peptide corresponding to the N-terminus of Akt3/PKB-alpha |
Host/Ig Type:
|
rabbit polyclonal IgG |
Purification:
|
peptide affinity purification |
Quality Control:
|
by western blot (see "Specificity") |
Liquid Carrier:
|
phosphate buffered saline (pH 7.4) containing 0.02% sodium azide |
Storage:
|
-20˚C |
Stability:
|
2 years |
1. Chan T et al (1999) Annu. Rev. Biochem. 68, 965-1014.
2. Cho H et al (2001) J. Biol. Chem, 276: 38349-38352.
3. Jones PF et al, (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171.
4. Cross DA et al (1995) Nature 378: 758.
5. Andjelkovic M et al (1996) Proc. Natl. Acad. Sci. U.S.A 93: 5699
6. Masure S et al (1999) Eur J Biochem 265: 353-360.
7. Nakatani K et al (1999) J Biol. Chem., 274: 21528-21532.
.
Western immunoblot:
LoVo cell lysates probes with purified Anti-AKT3 (Cat. #ABP-6007; lane 1) Anti-AKT3 with blocking peptide (lane 2); arrow indicates AKT3