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Description Catalog # Vial Size Price (USD)
Anti-Human AKT3 (NT), polyclonal ABP-6007 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
Western Blot:
0.5 -2.0 micrograms/ml
3-5 micrograms/sample derived from 1 x 10E6 cells
0.1-1 micrograms/ml
human, rat, mouse

reacts specifically with Akt3 at ~48 kDa; immunoreactive protein from LoVo, HeLa, or 3T3 cell lysates is detected by immunoblot; band is abolished by the addition of the immunizing Akt3 peptide

synthetic peptide corresponding to the N-terminus of Akt3/PKB-alpha
Host/Ig Type:
rabbit polyclonal IgG
peptide affinity purification
Quality Control:
by western blot (see "Specificity")
Liquid Carrier:
phosphate buffered saline (pH 7.4) containing 0.02% sodium azide
2 years
About AKT
General References

1. Chan T et al (1999) Annu. Rev. Biochem. 68, 965-1014.
2. Cho H et al (2001) J. Biol. Chem, 276: 38349-38352.
3. Jones PF et al, (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171.
4. Cross DA et al (1995) Nature 378: 758.
5. Andjelkovic M et al (1996) Proc. Natl. Acad. Sci. U.S.A 93: 5699
6. Masure S et al (1999) Eur J Biochem 265: 353-360.
7. Nakatani K et al (1999) J Biol. Chem., 274: 21528-21532.


Western immunoblot:

LoVo cell lysates probes with purified Anti-AKT3 (Cat. #ABP-6007; lane 1) Anti-AKT3 with blocking peptide (lane 2); arrow indicates AKT3