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Description Catalog # Vial Size Price (USD)
Anti-Human BARD1 (exon 4), polyclonal ABP-6056 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
use 1 to 4 ug/100 ug cell lysate
Western Blotting:
dilute 1:1,000 - 1:5,000
human; other species not tested

recognizes BARD1 at ~85 kDa in western blots of HeLa cell nuclear extracts; mouse BARD1 shares 21 of 27 amino acid residues within the immunizing sequence

synthetic peptide corresponding to a portion of human BRCA1 associated Ring Domain 1 encoded within exon 4 (LocusLink ID 580)
Host/Ig Type:
rabbit polyclonal IgG
affinity purified using the immunizing peptide immobilized on solid support
Quality Control:
by Western blotting (see "Specificity")
Liquid Carrier:
1 mg/ml antibody in Tris-citrate/phosphate buffer, pH 7 to 8; 0.1% sodium azide
1 year
About BARD1

Western Blot and IP-Western: A. Nuclear extracts from HeLa cells or U2OS cells snf B. U2OS cells expressing endogenous (-) or overexpressed (+) BARD1 probed with Anti-Human BARD1 (exon 4), polyclonal (Cat. #ABM-6056) C. Immunoprecipitation of nuclear extract from HeLa cells or control using normal rabbit IgG and susequently visualized with the antibody

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