Copyright © 2005 Cascade BioScience, Inc. All rights reserved.
Description | Catalog # | Vial Size | Price (USD) |
Anti-Human BRCA1 (exon 26), polyclonal | ABM-6057 | 100 ug | $295 |
Immunoprecipitation:
|
use 1 to 4 ug/100 ug cell lysate |
Western Blotting:
|
dilute 1:1,000 - 1:5,000 |
|
Reactivity:
|
human; other species not tested |
Specificity:
|
recognizes BRCA1 at ~220 kDa in western blots of HeLa cell nuclear extracts |
Immunogen:
|
synthetic peptide corresponding to a portion of human Breast Cancer Gene 1 encoded within exon 26 (LocusLink ID 580) |
Host/Ig Type:
|
rabbit polyclonal IgG |
Purification:
|
affinity purified using the immunizing peptide immobilized on solid support |
Quality Control:
|
by Western blotting (see "Specificity") |
Liquid Carrier:
|
1 mg/ml antibody in Tris-citrate/phosphate buffer, pH 7 to 8; 0.1% sodium azide |
Storage:
|
4˚C |
Stability:
|
1 year |
Western Blot and Immunoprecipitation: Nuclear extracts from HeLa cells probed with Anti-Human BRCA1 (exon 26), polyclonal (Cat. #ABM-6057) and Immunoprecipitation of nuclear extract from HeLa cells with the antibody or pre-immune rabbit IgG