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Description Catalog # Vial Size Price (USD)
Anti-Human Phospho-BRCA1 (pSer1189), polyclonal ABP-6058 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
Western Blotting:
dilute 1:1,000 - 1:5,000
Reactivity:
human; other species not tested
Specificity:

recognizes pBRCA1 in western blots of HeLa cell nuclear extracts

Immunogen:
phosphorylated synthetic peptide (pSer1189) corresponding to a portion of human Breast Cancer Gene 1
Host/Ig Type:
rabbit polyclonal IgG
Purification:
antibodies that were not phospho-specific were removed by solid phase adsorption; specific antibodies were affinity purified using the immunizing phospho-peptide immobilized on solid support
Quality Control:
by Western blotting (see "Specificity")
Liquid Carrier:
1 mg/ml antibody in Tris-citrate/phosphate buffer, pH 7 to 8; 0.1% sodium azide
Storage:
4˚C
Stability:
1 year
About BRCA1

Western Blot: 25 ug nuclear extracts from control (-) and (+) irradiated (10 Gy IR) HeLa cells probed with Anti-Human phospho BRCA1 (pSer1189), polyclonal (Cat. #ABM-6058)

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