Literature Request | Mailing List | E-Mail New Product Newsletter | Lab Protocols and Procedures
Product Application References | Technical Support | Customer Service | How to Order
Contact Us 

Copyright © 2004 Cascade BioScience, Inc. All rights reserved.

Description Catalog # Vial Size Price (USD)
Anti-Human FANCA, clone 5G9 ABM-6202 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
IP-Western:
10 microliters per 2 mg/ml sample to IP FANCA from cell lysates; subsequently visualize by immunoblot with polyclonal anti-FANCA (ABP-6201; 1:300-1:1000)
Western Blot:
antibody will detect endogenous FANCA in IPs and immunoblots of HeLa nuclear extract
Reactivity:
human
Specificity:
recognizes human FANCA at 163 kDa
Immunogen:
synthetic peptide corresponding to amino acids 1436-1455 of human FANCA
Host/Ig Type:
mouse monoclonal IgG1
Purification:
protein G chromatography
Quality Control:
by immunoblot on HeLa cell nuclear extracts
Liquid Carrier:
phosphate buffered saline
Storage:
-20˚C
Stability:
2 years
IP-Immunoblot: Cell lysates from wildtype (HSC93), FA-A (HSC72) and FA-F (EUFA698) lymphoblasts were Immunoprecipitated with monoclonal anti-FANCA (clone 5G9; aa 1436-1455; Cat. #ABM-6202). FANCA was subsequently visualized using polylconal antiFANCA (Cat. #ABP-6201)
About FANC
Application References

de Winter, J.P., van Der Weel, L., de Groot, J., Stone, S., Waisfisz, Q., Arwert, F., Scheper R. J., Kruyt, F. A., Hoatlin, M. E. and Joenje, H. “The Fanconi anemia protein FANCF forms a nuclear complex with FANCA,FANCC and FANCG”. Hum. Mol. Genet. 9:2665-74, 2000.

Waisfisz, Q., de Winter, J. P., Kruyt, F. A. E., de Groot, J., van der Weel, L., Dijkmans, L. M., Zhi, Y., Arwert, F., Scheper, R. J., Youssoufian, H., Hoatlin, M. E. and Joenje, H. “A physical complex of the Fanconi anemia proteins FANCG/XRCC9 and FANCA” PNAS (USA) 96: 10320–10325, 1999.

Meetei, A. R., Sechi, S., Wallisch, M., Yang, D., Young, M. K., Joenje, H., Hoatlin, M. E. and Wang, W “A Multiprotein Nuclear Complex Connects Fanconi Anemia and Bloom Syndrome” MCB 23:3417-3426, 2003

enlarge image