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Copyright © 2004 Cascade BioScience, Inc. All rights reserved.

Description Catalog # Vial Size Price (USD)
Anti-Human FAZF, polyclonal --COMING SOON! ABP-6211 100 ul $295
Research Applications
Antibody Characteristics
IP-Western Blot:
IP FAZF from cell lysates; immunoblot with a 1:500 -1:1000 dilution
recommended; see figure
recognizes FAZF at 53 kDa
GST-fusion protein corresponding to amino acids 114-295 of human FAZF
Host/Ig Type:
rabbit IgG
whole antiserum
Quality Control:
WB of immunoprecipitated Flag-tagged FAZF isolated from transfected cells
2 years
IP-Immunoblot: Lysates from 293 cells expressing FLAG-tagged FAZF (FAZF/pCEPFLAG)- and pCEP-FLAG-transfected cells as mock controls were subjected to SDS-PAGE electrophoresis and probed with anti-FAZF, polyclonal (ABP-6211) (A) or anti-FLAG (B). (Dai et al., JBC 277:26327, 2002)
About FAZF
Immunofluorescence: FAZF associates with sites of DNA synthesis in human hematopoietic progenitors. The first two rows show representative CD34 cells (1 and 2) grown for 3 days in the presence of erythroid growth factors and stained with a nuclear marker (DAPI), anti-BrdUrd (BrdU), and anti-FAZF (ABP-6211). 1a, DAPI; 1b, anti-BrdUrd; 1c, anti-FAZF; 1d, merged image of anti-BrdUrd and anti-FAZF; 2a, DAPI; 2b, anti-BrdUrd; 2c, anti-FAZF; 2d, overlap of anti-BrdUrd and anti-FAZF; 3, cells stained with DAPI and preimmune rabbit serum; 4, cells stained with anti-BrdUrd and DAPI; 5, cells stained with anti-FAZF and DAPI. (Dai et al., JBC 277:26327, 2002)
Application References

Hoatlin, M. E., Zhi, Y., Ball, H., Silvey, K., Melnick, A., Stone, S., Arai, S., Hawe, N., Owen, G., Zelent, A. and Licht, J. D. “A Novel BTB/POZ Transcriptional Repressor Protein Interacts With the Fanconi Anemia Group C Protein and PLZF” Blood 94:3737-47, 1999.

Dai, M.-S.,Chevallier, N., Stone, S., Heinrich, M. C., McConnell, M., Reuter, T., Broxmeyer, H. E., Licht, J. D., Lu, L. and M. E. Hoatlin “The Effects of the Fanconi Anemia Zinc Finger (FAZF) on Cell Cycle, Apoptosis and Proliferation Are Differentiation Stage-specific” JBC 277:26327, 2002

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