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Copyright © 2004 Cascade BioScience, Inc. All rights reserved.

Description Catalog # Vial Size Price (USD)
Anti-Human IRS-1 (308-315), polyclonal ABP-6021 100 ug $295
Research Applications
Antibody Characteristics
InfoSheet PDF
Western Blot:
0.5 -2.0 micrograms/ml
Immunoprecipitation:

3-5 micrograms/sample derived from 1 x 107 cells

ELISA:
0.1-1 micrograms/ml
Reactivity:
human; predicted mouse, rat
Specificity:

specific for human IRS-1 immunoreactive protein was detected from 3T3 cell lysate; band is abolished by the depletion of the specific synthetic peptide

Immunogen:
synthetic peptide (ITATSPAS) corresponding to amino acids 308-315 of human IRS-1 which contains the phosphorylation site of Serine 312
Host/Ig Type:
rabbit polyclonal IgG
Purification:
peptide affinity purification
Quality Control:
by western blot (see "Specificity")
Liquid Carrier:
200 ul phosphate buffered saline (pH 7.4) containing 0.02% sodium azide
Storage:
-20˚C
Stability:
2 years
About IRS
General References

1. Sun XJ et al (1991) Nature 352: 73-77
2. Sun XJ et al (1995) Nature 377: 173-177
3. Mothe I et al (1996) J Biol. Chem. 271: 11222-11227
4. Lavan BE et al (1997) J Biol. Chem. 272: 11439-11443
5. Lavan BE e al (1997) J Biol. Chem. 272: 21403-21407
6. Liu YF et al (2001) J Biol. Chem. 276 (17): 14459-14465
7. White MF (1998) Mol. Cell. Biochem. 182: 3-11