Copyright © 2004 Cascade BioScience, Inc. All rights reserved.
| Description | Catalog # | Vial Size | Price (USD) |
| Anti-Human Phospho-IRS-1 (pSer312), polyclonal | ABP-6020 | 100 ug | $295 |
|
Western Blot:
|
0.5 -2.0 micrograms/ml |
|
Immunoprecipitation:
|
3-5 micrograms/sample derived from 1 x 107 cells |
|
ELISA:
|
0.1-1 micrograms/ml |
|
Reactivity:
|
human, mouse; predicted rat |
|
Specificity:
|
specific for human IRS-1 phosphorylated at serine 312; does not recognizes other phosphorylated sites or non-phosphorylated IRS-1 or IRS-2; band is abolished by the depletion of the phosphorylated specific synthetic peptide |
|
Immunogen:
|
synthetic peptide (ITATpSPAS) corresponding to amino acids 308-315 of human IRS-1 at the phosphorylated site of Serine 312 |
|
Host/Ig Type:
|
rabbit polyclonal IgG |
|
Purification:
|
peptide affinity purification |
|
Quality Control:
|
by western blot (see "Specificity") |
|
Liquid Carrier:
|
phosphate buffered saline (pH 7.4) containing 0.02% sodium azide |
|
Storage:
|
-20˚C |
|
Stability:
|
2 years |
1. Sun XJ et al (1991) Nature 352: 73-77
2. Sun XJ et al (1995) Nature 377: 173-177
3. Mothe I et al (1996) J Biol. Chem. 271: 11222-11227
4. Lavan BE et al (1997) J Biol. Chem. 272: 11439-11443
5. Lavan BE e al (1997) J Biol. Chem. 272: 21403-21407
6. Liu YF et al (2001) J Biol. Chem. 276 (17): 14459-14465
7. White MF (1998) Mol. Cell. Biochem. 182: 3-11
Immunoprecipitation:
Activated 3T3cell lysate immunoprecipitated with Anti-Human Phospho-IRS-1 (pSER312) and probed with the same antibody.