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Description Catalog # Vial Size Price (USD)
Anti-Human Phospho-Progesterone Receptor (Ser294; clone 608) ABM-4203 200 ug $295
Research Applications
Antibody Characteristics
Western Immunblotting:
10 micrograms/ml
10 micrograms per sample
Supershift (EMSA):
under evaluation
human, other species in test
detects phosphorylated PR predominantly at 115 kDa (B-isoform)
phosphopeptide; human sequence: amino acids 287-300 (pSer294)
Host/Ig Type:
mouse monoclonal IgG
protein G chromatography
Quality Control:
by WB on cytosolic and nuclear extracts of T47D cells treated and untreated with a synthetic progestin agonist
Liquid Carrier:
phosphate-bufferd saline:azide
1 year
About Progesterone R
InfoSheet PDF
Immunoprecipitation Analysis
100 ug whole cell extracts of T47D cells treated (+) and un-treated (-) with R5020 (synthetic progestin agonist) were IP'd with anti-pPR (Ser294; clone 608) linked to protein A-Sepharose-mouse IgG conjugate; subsequent WB analysis effected using Anti-PR-NT, clone 1294
Immunoblot Analysis
Cytosolic (C) and Nuclear (N) extracts of T47D cells treated (+) and un-treated (-) with R5020 (synthetic progestin agonist); primary antibody is anti-pPR (Ser294; clone 608; 4 ug/ml)
General References
Knotts, TA,Orkiszewski, R.S., Cook, R.G., Edwards, D.P.,Weigel, N.L. J. Biol. Chem. 276:8475-8483, 2001.

Zhang, Y, Beck, C.A., Poletti, A, Edwards, D.P., Weigel, N.L. Molecular Endocrinology 9: 1029-1040, 1995.

Zhang,Y., Beck, C.A., Poletti, A, Clement, J.P, Prendergast, P., Edwards, D.P,Weigel, N.L.Molecular Endocrinology 11: 823-832, 1997.

Takimoto,G.S. Hovland, A.R., Tasset, D.M, Melville, M.Y., Tung, L, Horwitz,K.B. J.Biol.Chem. 271:13308-13316, 1996.

Clemm, D.L., Sherman, L., Boonyaratanakornkit, V, Schrader,W.T.,Weigel, N.L., Edwards, D.P.Molecular Endocrinology 14: 52-65, 2000.

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