Literature Request | Mailing List | E-Mail New Product Newsletter | Lab Protocols and Procedures
Product Application References | Technical Support | Customer Service | How to Order
Contact Us 

Copyright © 2004 Cascade BioScience, Inc. All rights reserved.

Description Catalog # Vial Size Price (USD)
Anti-Ski, monoclonal (clone G8) ABM-3001 100 ug $295
Research Applications
Antibody Characteristics
Western Immunblotting:
1-5 micrograms/ml
1-4 micrograms/sample
1-10 micrograms/ml
human, mouse, rat, chick, Xenopus
detects cellular Ski at ~95 kDa and hyperphosphorylated forms ranging to 115 kDa
v-Ski fusion protein
Host/Ig Type:
mouse monoclonal IgG
protein G chromatography
Quality Control:
by WB using rat cells infected with pBABEpuro hSki expressing full-length human Ski proteins
Liquid Carrier:
phosphate-bufferd saline:0.05% sodium azide
2 years
InfoSheet PDF
About Ski

Photomicrograph illustrates fibroblast expression of Ski; stained effected with Cascade Bioscience Ski antibody (catalog number ABM-3001).
Immunoblot Analyses

1. Rat cells infected with pBABEpuro hSki expressing full length human Ski proteins of ~95 kDa and 100 kDa (100 kDa is the hyperphosphorylated form of 95 kDa)

2. Rat cells infected with pBABEpuro vector showing endogenous Ski proteins ranging from ~97 kDa to 115 kDa

Blot probed with G8 IgG1 at 1 microgram/ml.
General References
1. Stavnezer et al., (1989) MCB 9: 4038-4045
2. Colmenares et al., (1991) J.Virol. 65: 4929-4935
3. Zheng, N. et al., (1999) Cell. Growth Differ. 10(4): 243-254
4. Sun, Y. et al., (1999) Mol. Cell 4(4): 499-509
5. Luo, K. et al., (1999) Genes Devel. 13: 2196-2106
6. Akiyoshi, S. et al., (1999) J. Biol. Chem. 274: 35269-35277
enlarge image
enlarge image